Now, 50 mg of prepared powder was dissolved again with 10 ml of methanol/water (8:2, v/v) and extracted for 30 min under ultrasound. After centrifugation at 13,000 rpm for 10 min at 4C and filtration through a 0.22-μm filter, 1.0 μL of filtrate was injected to UHPLC-QTOF MS for a qualitative analysis.
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